GLK gel PrG Affinity Media
GLK gel PrG affinity media is recombinant Protein G ligand bound on a cross-linked agarose-gel substrate. Protein G can attach to the Fc region of various mammalian IgG, while the Fab region can still link to antigens, so it is useful for the separation of immune complexes. Protein G and Protein A have different IgG binding sites, depending on the source of Protein G. Different from Protein A, Protein G can bind polyclonal IgG more strongly. Also, Protein G can also bind rat IgG, hIgG3 and mouse IgG1, thus improving the downstream process of antibody purification.
Parameters
Substrate: high cross-linked agarose
Ligand: Recombination Protein G
Particle Size: 90 µm
Dynamic Capacity: 20 mg hIgG/mL-resin
Max Pressure: <1.5 bar
pH Stable Range: 2-9
CIP Condition: 8M carbamide, 6M guanidine hydrochloride
Temp.: 4-40 ºC
Storage: 4-8 ºC 20% EtOH
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