China Anti -Pcna Rabbit Polyclonal Primary Antibody

Description
PCNA rabbit polyclonal
Protein full name
Proliferating cell nuclear antigen
Synonyms
PCNA, ATLD2, proliferating cell nuclear antigen
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Isotype
IgG
Purity
Affinity purification
Predicted MW.
33 kDaObserved MW.

33 kDa

Applications

ApplicationsWBIHC

IF

Human, Mouse, Rat

1:2000

1:300-1:1000

1:200-1:500

Liver, lung, spleen

Background

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Images

Western blot analysis of PCNA (GB11010) at dilution of 1:2000.

Lane 1: hela cell lysate

Lane 2: Jurkat cell lysate

Lane 3: Raw264.7 cell lysate

Lane 4: NRK-52E cell lysate

Lane 5: Human heps tissue lysate

Immunohistochemistry analysis of paraffin-embedded human liver cancer using PCNA (GB11010) at dilution of 1:500.

Immunohistochemistry analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1:500.

Immunohistochemistry analysis of paraffin-embedded rat spleen using PCNA (GB11010) at dilution of 1:500.

Immunofluorescence analysis of paraffin-embedded mice hela tumor using PCNA (GB11010) at dilution of 1:500.

Immunofluorescence analysis of paraffin-embedded mouse lung using PCNA (GB11010) at dilution of 1:200.

Storage

Storage
Store at -20 ºC for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

Certificates & Exhibition:

Package & Delivery
Q/A
1. Are you manufacturer?

Yes we are, and we can offer the best price for you.

2. Payment Terms:

We can accept 30% advance and before ship the goods to port pay the balance.

3. How about the quality?

We can offer the samples to you with free, you can check the quality before place the order.

4. How do you treat quality complaint?
First of all, our quality control will reduce the quality problem to near zero. If there is a quality problem caused by us, we will send you free goods for replacement or refund your loss.
5. Could you offer a free sample for the quality test?
Free sample is available for customers.
about us:

Wuhan Servicebio Technology Co., Ltd. was founded in 2007, headquartered in Wuhan Optical Valley Biological City, is a high-tech enterprise specializing in providing technical services and scientific research products for life and health science research. With more than 1000 employees and more than 40 service centers in large and medium-sized cities across the country, it can provide support and service to scientists who explore the mysteries of life in an all-round way.

The company has strong scientific research and service strength, has antibody research and development and production center, ultra-micro pathology center, pathological detection center, SPF experimental animal center and other technical platforms, (the company also) has a complete set of experimental platforms and instruments and equipment for pathological immune detection and analysis, and mastered the technical core and patented technology, and independently developed and optimized a variety of experimental methods and processes, and developed a number of matching reagents and consumables. The company has a special R & D team to produce and verify antibodies .Laboratory consumables centrifugal tubes and suckers have formed large-scale production and can be automated packaged. We can provide experimental design, method optimization, Experimental operation, analysis and testing, reagent consumables and other omni-directional integrated scientific research and technical services for customers.

The company's experimental instruments have passed the system certification, not only the internal introduction of ISO9001 quality management system, but also through the European Union CE security certification and FCC certification, National High-tech Enterprise Certification and so on.

Servicebio independent research and development laboratory instruments and matched consumables have been widely used in more than 200 cities in China. The products have been exported to the United States, Canada, Germany, the United Kingdom, Singapore, Malaysia, Thailand and other countries and regions.
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