Products Name
RT-PCR Detection Kit
Size
48 tests/kit, 96 tests/kit
Advantages
(1) 3 Target detection: Qualitative detection of ORF 1ab gene, N gene
(2) Internal reference monitoring: Effectively monitor the entire detection process such as extraction, reverse
transcription and amplification to avoid false negative results
(3) Anti-pollution system: UNG enzyme and dUTP are used to effectively prevent the contamination of amplification products and avoid false positive results
Products Information
(1) Packaging speifications: 48 servings 1 box, 96 servings / box
(2) Applicable samples: nasopharyngeal swabs, throat swabs, sputum or alveolar lavage fluid, etc.
(3) Amplfication time: about 1 hour and 20 minutes
(4) Sensitivity: 300 copies 1 mL
(5) Precision: Ct value variation coefficient CV within / between batches are less than 5%
(6) Specificity: No cross-reativity with other related pathogens that are the same or similar to the infection site
(7) Applicable instrument: ABI 7500, LightCycler480, SL AN96, LM2012, etc.
(8) Reagent validity:-15 C ~ -25 C, 12 months
Principle Of Detection
This product is a fluorescent probe-based Taqman RT-PCR assay system.
Firstly, the RNA of 19-C0v will be reverse transcribed into DNA by reverse transcriptase, and then PCR amplification will be performed with cDNA astemplate.
During amplification of the template, the Taqman probe will be degraded due to the 5'-3' polymerase activity
and exonuclease activity of Taq DNA polymerase, then the separation of fluorescent reporter and quencher enables the fluorescent signal to be detected by instrument.
The ORF1ab gene of 19-C0V will be detected qualitatively by FAMchannel, the N gene of 19-C0V will be detected qualitatively by JOE channel, the E gene of 19-C0V will bedetected qualitatively by ROX channel, and the internal reference will be detected by CY5 channel.dUTP and UNG enzyme are used in the kit to prevent contamination of the amplified products.
Internal reference is used in the kit for quality control starting from sample collection.
Medical Test Kit Novel Detection Kit with Fluorescence PCR Method.
ATTENTION
(1) The product is not colloidal gold. (Fluorescent PCR)
(2)Medical diagnostic kits is not for home use and requires professional medical personnel to use it in a hospital or laboratory
(3) Need to be used with professional PCR instruments, please refer to the manual
(4) Before using nucleic acid detection, it is necessary to take calibration acid from a sample (such as a throat swab). There are two different methodological extraction reagents to choose from. One is the magnetic bead method for nucleic acid extraction reagents, which is used in conjunction with our extraction equipment (the method is easy to operate).