Sepromax® A40 plus
Sepromax® A40 plus designed for the isolation and purification of monoclonal antibodies. Compared with traditional agarose media, Sepromax® A40 plus has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. 0.1-0.5M NaOH can uses for clean-in-Place (CIP).
Sepromax® A40 plus is recombinant protein A (rProtein A) immobilized on the surface of macro-porous PS/DVB microsphere substrate. rProtein A has better alkali-resisting that ensures good stability in high pH conditions. With GLK® hydrophilic treatment and binding technology, PS/DVB surface removes non specific binding with good combination of rProtein A ligand. Hence, Sepromax® A40 plus is extremely useful for isolating of monoclonal antibodies.
Advantages:
• High rigidity, low backpressure, suitable for large-scale downstream processing.
• Outstanding high dynamic binding capacity at low residence time reduces
process time and amount of medium used.
• Excellent alkali resistance, 0.1-0.5 M NaOH for CIP.
• Long lifetime, low ligand leakage.
Main Characteristics
Support Matrix: Poly(styrene/divinylbenzene) (PS/DVB)
Ligand: Recombinant Protein A
Particle size: 40µm
Dynamic Binding Capacity (DBC): Approx. 40 mg human IgG/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 5 cm, Residence time 0.6 min)
Shrinkage/Swelling: < 1% from 1-100% organic solvent
pH range (Long term): pH 2-10
Maximum Operating Pressure: 1500 psi (100 bar / 10 MPa)
Cleaning Agents: 0.1-0.5M NaOH
Temperature Stability: 4-40 °C
Storage: 20% ethanol (2-8ºC)
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