China Bio&Media Blood Urine Nitrogen Test Kit Ivd Reagents

The blood urine nitrogen (BUN) assay kit is intended for the accurate and direct quantitative measurement of BUN content in human plasma.
Test principle
In the coupling reaction above, urea is hydrolyzed in the presence of urease, producing NH3 and CO2. And then NH3 reacts with α-KG, generating glutamic acid. In the meanwhile, NADH is oxidized and converted into NAD+.
The concentration of BUN can be calculated from the decreasing rate of absorbance detected at a wavelength of 340 nm.
Product Features

1. Linear range of BUN activity: [0.3, 33.7] mmol/L

Linear correlation coefficient ∣r∣≥ 0.990
Linear deviation ≤ ± 10.00%

2. Absorbance of blank ≥ 0.90

3. Changing rate of absorbance of blank ΔA/min ≤ 0.02

4. Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.50 while testing the specific BUN sample of concentration of 16.7 mmol/L.

5. Accuracy

Relative deviation is within ±10%.

6. Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.
Technical Parameters

Temperature37ºCOptical path length (OPL)1.0 cmMain wavelength340 nmSecondary wavelength450 nm (Optional)MethodVelocity methodReaction directionNegativeSample10 μLR1200 μLR250 μLDelay time50 sReaction time180 s

Reference range of ALT activity: 3.0 - 6.4 mmol/L.
Packing Specifications
R1: 2x60 ml, R2: 2x15 ml
R1: 4x60 ml, R2: 4x15 ml
R1: 1x40 ml, R2: 2x10 ml (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.