Biomedia total bile acid(TBA) assay kit is applied to measure the concentration of TBA in human serum or plasma.
Testing Principle
The determination of total bile acid(TBA) concentration is based on enzyme cycling method, which allows for the signal amplification through cycled regeneration reactions. In the forward reaction, bile acid molecules are oxidized by thio-NAD+, generating oxidized bile acids and reduced co-enzyme thio-NADH. While in the reverse reaction, the oxidized bile acids are reduced back to bile acids by the excess co-enzyme NADH in the presence of 3-α-hydroxysteroid dehydrogenase(3-α-HSD), and are ready for the next round of forward oxidation reaction. The signal amplification by this enzyme cycling method enables a much more higher detection sensitivity of the test. The concentration of bile acid can be calculated from the rate of thio-NADH formation, which is detected at 405 nm.
Product FeaturesLinearity range: 0 - 180 μmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.Absorbance of blank ≤ 0.90Changing rate of absorbance of blank (ΔA/min) ≤ 0.05Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.30 while testing the specific sample of concentration of 50 μmol/L.Accuracy: Relative deviation is within ±10%. Precision: intra assay CV(%) ≤ 5%; inter assay CV(%) ≤ 6%.
Technical Parameters
Main WavelengthSecondary WavelengthMethodReaction DirectionSample VolumeR1 VolumeR2 VolumeReaction TimeReaction Time405 nm600 nm (optional)Velocity methodPositive4 μl240 μl80 μl60 s180 s
Reference range: 2 - 12 μmol/L
Packaging Specification
R1: 2x60 ml, R2: 2x20 ml
R1: 4x60 ml, R2: 4x20 ml
Storage and Period of Validity
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.