China Bio&Media Total Bilirubin Test Kit Ivd Reagents Hospital Lab Hepatic Disease High Sensitivity

Total Bilirubin (T-Bil) assay kit is applied to measure the concentration of T-Bil in human serum or plasma.
Testing Principle
In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
Product Features

1. Linear range of T-Bil level: [1, 684] μmol/L

Linear correlation coefficient ∣r∣≥0.990
Linear deviation ≤ ± 10.00%

2. Absorbance of blank ≤ 0.10

3. Sensitivity

The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.

4. Accuracy

Relative deviation is within ±10%.

5. Precision

Intra CV% ≤ 5.0%; inter CV% ≤5.0%.
Technical Parameters

Temperature37ºCOptical path length (OPL)1.0 cmMain wavelength450 nmSecondary wavelength546 nm (Optional)MethodEnd-point method
(dual reagents)Reaction directionNegativeSample8 μLR1240 μLR260 μLReaction time300 s

Reference range: 3.42 - 20.50 μmol/L
Packing Specifications
R1: 2x60 ml, R2: 2x15 ml
R1: 4x60 ml, R2: 4x15 ml
R1: 1x40 ml, R2: 1x10 ml (Sample)
Storage and Stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.