The HDL-C assay kit is intended for the direct quantitative measurement of high density lipoprotein cholesterol in human serum.
Testing Principle
This assay kit is applied to test the HDL-C levels based on PPD (polyanion polymer/ detergent) method. During the reaction, LDL, VLDL and chylomicrons are suppressed by the polyanion polymer and detergent and can not react with cholesterol oxidase reagent. In the meanwhile, HDL-C is dissolved by the surfactant, resulting in the release of cholesterol and reaction with enzymes. The concentration of HDL can be calculated by the change of absorbance of the serum sample.
Product FeaturesLinearity range: 0.50 - 3.90 mmol/L; linear correlation coefficient ≥0.990; linearity deviation is within ±10%.Absorbance of blank ≤ 0.10Sensitivity: the difference of absorbance per unit of concentration (ΔA) ≥ 0.01 while testing the specific sample of concentration of 1.87 mmol/L.Accuracy: Relative deviation is within ±10%. Precision: intra assay CV(%) ≤ 5%; inter assay CV(%) ≤ 5%.
Technical Parameters
Main WavelengthSecondary WavelengthMethodReaction DirectionSample VolumeR1 VolumeR2 VolumeReaction Time600 nm700 nm (optional)End point methodPositive3 μl240 μl80 μl300 s
Reference range: 1.19 - 1.69 mmol/L
Packaging Specification
R1: 2x60 mL, R2: 2x20 mL
R1: 4x60 mL, R2: 4x20 mL
R1: 1x30 mL, R2: 1x10 mL (Sample)
Calibrator: additional purchase
Quality control: additional purchase
Storage and Period of Validity
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.